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Matthew
L. Rise
Shaw Assistant Scientist
Great Lakes WATER Institute, UWM
Phone: (414)
382-1734
Fax: (414)
382-1705
E-Mail:
Web
Site: WATER
Web Page
Great
Lakes WATER Institute
600 E. Greenfield Ave.
Milwaukee, WI 53204 |
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Research Interests
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For
the past few years, I have helped develop genomic resources (e.g.
EST databases, cDNA and oligonucleotide microarrays) for salmonid
research. DNA microarrays allow the researcher to analyze relative
expression levels of thousands of genes simultaneously. I have used
these tools to study variability of gene expression within and between
salmonid species, transcriptome dynamics over rainbow trout embryogenesis,
and the responses of salmonid transcriptomes to various insults (e.g.
rickettsial infection, pulp mill effluent exposure). Currently in
the lab, these genomic resources are being used to identify embryonic
trout molecular biomarkers of organophosphate pesticide (OP) exposure.
OPs exert their toxic effects by inhibiting acetylcholinesterase,
an enzyme critical to the normal functioning of cholinergic synapses.
In my lab, various techniques are used to assess the impact of OP
exposure on salmonid morphogenesis and behavior. Other potential
stressors under similar evaluation in the lab include heavy metals,
various anthropogenic effluents (and individual contaminants therein),
and diets of varying nutritional quality. We further develop salmonids
as models for studying the effects of environmental toxins on human
health. | |
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Education
| • | Whitworth
College, BS Biology, 1988 |
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| • | Boston
College, MS Molecular Genetics, 1990 |
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| • | University
of Victoria, PhD Developmental Biology, 2001 |
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| • | Centre for Biomedical Research,
U of Victoria, PDF Genomics, 2003 |
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Selected Publications
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von Schalburg KR, McCarthy SP, Rise ML, Hutson JC, Davidson
WS, and Koop BF. 2006. Expression of morphogenic genes in mature ovarian
and testicular tissues: Potential stem-cell niche markers and patterning
factors. Molecular Reproduction and Development 73:142-152.
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Ng SH, Artieri CG, Bosdet IE, Chiu R, Danzmann RG, Davidson WS, Ferguson
MM, Fjell CD, Hoyheim B, Jones SJ, de Jong PJ, Koop BF, Krzywinski MI, Lubieniecki
K, Marra MA, Mitchell LA, Mathewson C, Osoegawa K, Parisotto SE, Phillips
RB, Rise ML, von Schalburg KR, Schein JE, Shin H, Siddiqui
A, Thorsen J, Wye N, Yang G, and Zhu B. 2005. A physical map of the genome
of Atlantic salmon, Salmo salar. Genomics 86:396-404.
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von Schalburg KR, Rise ML, Cooper GA, Brown GD, Gibbs AR,
Nelson CC, Davidson WS, and Koop BF. 2005. Fish and chips: various methodologies
demonstrate utility of a 16,006-gene salmonid microarray. BMC Genomics 6:126.
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von Schalburg KR, Rise ML, Brown GD, Davidson WS, and Koop
BF. 2005. A comprehensive survey of the genes involved in maturation and
development of the rainbow trout ovary. Biology of Reproduction 72:687-699.
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Rise ML, Jones SRM, Brown GD, von Schalburg KR, Davidson
WS, and Koop BF. 2004. Microarray analyses identify molecular biomarkers
of Atlantic salmon macrophage and hematopoietic kidney response to Piscirickettsia
salmonis infection. Physiological Genomics 20:21-35.
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Rise ML, von Schalburg KR, Brown GD, Mawer MA, Devlin RH,
Kuipers N, Busby M, Beetz-Sargent M, Alberto R, Gibbs AR, Hunt P, Shukin
R, Zeznik JA, Nelson C, Jones SRM, Smailus DE, Jones SJM, Schein JE, Marra
MA, Butterfield YSN, Stott JM, Ng SHS, Davidson WS, and Koop BF. 2004.
Development and application of a salmonid EST database and cDNA microarray:
data mining and interspecific hybridization characteristics. Genome
Research 14:478-490.
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Burke RD, Murray GA, Rise M, and Wang D. 2004. Integrins
on eggs: the bC integrin subunit is essential for formation of the
cortical actin cytoskeleton in sea urchin eggs. Developmental Biology 265:53-60.
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Rise M and Burke RD. 2002. SpADAM, a sea urchin ADAM,
has conserved structure and expression. Mechanisms of Development 117:275-281.
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Murray G, Reed C, Marsden M, Rise M, Wang D and Burke RD.
2000. The alphaBbeta C integrin is expressed on the surface of the
sea urchin egg and removed at fertilization. Developmental Biology 227:633-647.
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Rise ML, Frankel WF, Coffin JM, and Seyfried TN. 1991.
Genes for epilepsy mapped in the mouse. Science 253:669-673.
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